Application

This Anti-RNA Helicase A/Dhx9 Antibody, clone 8E3 is validated for use in western blotting & ICC for the detection of RNA Helicase A/Dhx9.

Research CategoryEpigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected RNA Helicase A/Dhx9 in NIH3T3 cell lysate (Kotani, M., et al. (2010). Hybridoma. 29(3):259-261).
Immunocytochemistry Analysis: A representative lot detected RNA Helicase A/Dhx9 in NIH3T3 cells (Kotani, M., et al. (2010). Hybridoma. 29(3):259-261).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

RNA Helicase A, also known as DEAH box protein 9 (mHEL-5), DHX9, or Nuclear DNA helicase II (NDH II), and encoded by the mouse gene Dhx9, is a component of the CRD-mediated mRNA stability complex. The CRD-mediated complex is a protein complex that binds to, and promotes stabilization of, mRNA molecules containing the coding region instability determinant (CRD). In humans, it consists of IGF2BP1 and at least four additional proteins: HNRNPU, SYNCRIP, YBX1, and RNA Helicase A. RNA Helicase A operates by unwinding dsDNA and RNA in a 3′ to 5′ direction. Additionally, RNA Helicase A acts as a transcriptional activator and interacts with LARP6 which is a protein that has been associated with fasting glucose traits, type 2 diabetes and obesity as well as the stabilization of collagen mRNAs for CO1A1 and CO1A2. RNA Helicase A is localized to the nucleolus within the nucleus but can also be found in the cytoplasm localized in cytoplasmic mRNP granules containing untranslated mRNAs and thus the protein shuttles between the nucleus and cytoplasm depending upon the microenvironment.

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of mouse RNA Helicase A/Dhx9.

Epitope: N-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RNA Helicase A/Dhx9 in 10 µg of HeLa nuclear extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~140 kDa observed